This study explores the dynamics of interorganizational relationships (IORs) among police departments in a region in Southwest, Virginia. We focus on the perceptions of jurisdictional/organizational and policy/procedure boundaries in regional IORs, closely following Giacomantonio’s (2014) typology of policing boundaries. Through a qualitative analysis of over 700 excerpts that resulted from 22 semi-structured interviews with law enforcement personnel, we identified significant themes of ‘bridging’ and ‘separation’ across these boundaries. Data revealed more discussion of bridging in jurisdictional/organizational boundaries (82%) compared to policy/procedure boundaries (60%). Findings suggest that bridging and separating at jurisdictional/organizational and policy/procedure boundaries is influenced by rank within law enforcement agencies. Variations in perceptions of IORs by rank align with fragmentation of police occupational culture within an agency (Reuss-Ianni 1983; Paoline 2003; Cohen 2018). We argue that our findings support the inclusion of boundary types in collaborative process frameworks and also add to understanding the complexities of law enforcement IORs when developing strategies to enhance and improve these efforts across varying ranks of law enforcement.